ABSTRACT
Background: Millions of people throughout the world have been impacted by the 2019 pandemic coronavirus disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (COVID-19). Critical risk factors for the severity and fatality rates of COVID-19 are bacterial co-infection and subsequent bacterial infection. It is also necessary to take into account the antibiotic resistant as a result of overuse. We will review the secondary bacterial infections and bacterial co-infections in COVID-19 patients in this study, we aimed To isolate Staphylococcus aureus and investigate the role of Panton-Valentine Leukocidin (PVL) toxin in severity of infection in COVID-19 patients. Study the antibiogram of Staph, aureus. Methods: This study was conducted with 100 patients of Iraqi-Kut city in both sexes, aged 18-70 years, in cooperation with AL-Zahraa Teaching Hospital in day 10 of COVID-19 infection. Collected 100 sputum samples (50 mild and 50 as severe), during period from December 2020 to June 2021, It was looked at whether individuals with the coronavirus disease of 2019 (COVID-19) had additional respiratory pathogens coinfected with them. Detection of bacterial strains by use VITEK2 system, detection of PVL gene and mec A gene by real time per technique. Results: In this study, collected 100 sputum samples from COVID-19 patients, the results showed that the percentage of bacterial infection (63%), fungal infection (16%), no growth (21%). percentage of staphylococcus aureus was (17%);detection of PVL gene as positive results (29.4%). Conclusions: simultaneous PVL gene and VITEK2 system identification of respiratory infections in SARS-CoV-2 patients, mec In particular during the COVID-19 pandemic, a gene by molecular diagnosis assays is required for determining the causal agents of coinfection. © 2023, ResearchTrentz Academy Publishing Education Services. All rights reserved.